Table 1.
Group A Streptococcus (GAS) Strains Used in This Study
| Strain | Description | Reference |
|---|---|---|
| MGAS2221 | A previously characterized serotype M1 GAS strain | [23] |
| PGAS951 | M11 strain from the CDC; isolated in 2016 from a blood specimen of a patient in CT; the patient did not have puerperal sepsis | This study |
| PGAS953 | M11 strain from the CDC; isolated in 2014 from a blood specimen of a patient in MN; the patient did not have puerperal sepsis | This study |
| PGAS955 | M11 strain from the CDC; isolated in 2017 from a blood specimen of a patient with puerperal sepsis in MN | This study |
| PGAS956 | M11 strain from the CDC; isolated in 2017 from a blood specimen of a patient with puerperal sepsis in NY | This study |
| PGAS958 | M28 strain from the CDC; isolated in 2015 from a blood specimen of a patient in CA; the patient did not have puerperal sepsis | This study |
| PGAS959 | M28 strain from the CDC; isolated in 2014 from a blood specimen of a patient in NY; the patient did not have puerperal sepsis | This study |
| PGAS961 | M28 strain from the CDC; isolated in 2013 from a blood specimen of a patient with puerperal sepsis in OR | This study |
| PGAS962 | M28 strain from the CDC; isolated in 2014 from a blood specimen of a patient with puerperal sepsis in NM | This study |
| MGAS6180 | A previously characterized serotype M28 GAS strain | [19] |
| M28.RD2SPEC | A derivative of MGAS6180 in which RD2 has been tagged with a spectinomycin-resistance cassette inserted between genes 1329 and 1330 | This study |
| M28ΔRD2-Kn | A derivative of MGAS6180 in which all copies of RD2 have been deleted, with a kanamycin-resistance cassette replacing them | This study |
| M28ΔRD2 | A derivative of M28ΔRD2-Kn in which the kanamycin resistance cassette was replaced with the natural RD2 integration site | This study |
| RD2Comp | A derivative of M28ΔRD2 in which RD2 from strain M28.RD2SPEC has been introduced | This study |
| M28ΔR28 | An r28 deletion mutant derivative of MGAS6180 | This study |
Abbreviations: CA, California; CDC, Centers for Disease Control and Prevention; CT, Connecticut; MN, Minnesota; NM, New Mexico; NY, New York; OR, Oregon.