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Purification of α-amylase from strain JSI.
| Steps | Total activity (Units) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude enzyme | 6708 | 1738 | 3.86 | 1.00 | 100 |
| Ammonium sulphate fraction 60–80% | 10.1 | 0.73 | 13.7 | 3.6 | 0.15 |
| DEAE Sepharose Fast Flow | 28.3 | 1.13 | 25.1 | 6.5 | 0.42 |
