Figure 4.

Tumor Growth Was Inhibited in DPDL1E-Vaccinated Mice

(A) Experimental workflow of the mouse preventive model. Mice were immunized subcutaneously three times at 14-day intervals. On day 7 after the third immunization, mice were injected s.c. with tumor cells. On day 7 after tumor challenge, the fourth immunization was administered. (B) Tumor volume measurements were performed to evaluate tumor growth. The endpoint was predefined by mouse death or a tumor reaching 2 cm in any direction. Statistically significant differences were determined by Mann-Whitney U test. (C) A mouse survival curve was constructed after the tumor challenge. Statistically significant differences were determined using a log rank test. (D) A scatterplot with tumor volumes on day 17 as the coordinates of the horizontal axis and serum antibody titers on day 7 after the third immunization as the coordinates of the vertical axis. The correlation coefficient was determined using GraphPad Prism 6 software. (E) The schedule for the metastatic mouse model. (F) Representative images of lung metastasis. (G and H) The total number of nodules (G) and the number of large nodules (H) in the lung. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.