The DPDL1E Vaccine Decreased the Ratio of PD-1+ Cells and Increased the Ratio of PD-L1+ Cells in Tumors
(A) PD-L1+ cells were stained with anti-CD274-APC and anti-CD45-FITC. (B) PD-1+ cells were stained with anti-CD279-APC and anti-CD45-FITC. (C) LAG3+ cells were stained with anti-CD223-PE, anti-CD3-FITC, and anti-CD8-PerCP-Cy5.5. (D) LAG3+PD-1+ cells were stained with anti-CD279-APC, anti-CD223-PE, anti-CD3-FITC, and anti-CD8-PerCP-Cy5.5. All samples were detected using FCM, and the data were analyzed using FlowJo software. *p < 0.05, **p < 0.01, ***p < 0.001. Statistically significant differences were assessed using Student’s t test.