Figure 5.

IL-33 inhibits IL-12 induced granzyme B expression in a nutrient deprived environment. (A) Serum deprived CD8 T cells were treated with 20 ng/ml IL-33 prior determination of abundance of ST2L⁺ CD8^low cells by flow cytometry. Representative flow cytometry histograms showing ST2L expression on CD8^low T cells treated with IL-33 compared to untreated control. Ctrl. = untreated starved CD8⁺ T cells. (B) Percentage of CD8 T cells expressing intracellular Granzyme B (GrzmB) upon treatment with IL-33 [20 ng/ml], IL-12 [5 ng/ml], or the combination of both. (C) Representative data showing the modulation of intracellular GrzmB expression by IL-33 and IL-12. qRT-PCR relative expression (2^−ΔCt) of (D) TBX21 (T-bet), (E) BLIMP-1, (F) GATA3, and (G) FOXP3. The expression levels were normalized to the housekeeping genes GAPDH and RPL13A. Data are shown as mean ± SD of (A) n = 7, (B) n = 4, (D,E) n = 4 and (F,G) n = 3 of different donors with at least n = 3 independently performed experiments. *,^#p ≤ 0.05, **p < 0,01, and ^###p < 0.001 using (A) Wilcoxon matched-pairs signed rank test, (B) Friedman test with Dunn's post-test, or (D–G) RM one-way ANOVA with Bonferroni's post-test. Hashtags (#) indicate significance compared to the untreated control, asterisks (*) indicate significance as indicated.