Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 15;42(3):1047–1056. doi: 10.3892/or.2019.7234

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Ray et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 4. — MIB1 is targeted by miR-198. (A) RT-qPCR results revealed lower MIB1 abundance in LNCaP and DU145 cells transfected with miR-198 mimic, relative to control mimic samples and normalized to GAPDH. (B) MIB1 protein in LNCaP and DU145 cells is reduced after miR-198 transfection compared with control mimic; representative blots are presented. β-actin was used as an endogenous control. (C) Predicted binding site of miR-198 within wild-type MIB1 3′UTR and mutated sequence used in luciferase assay. Location of miR-198 seed sequence denoted by blue text (AGACCUG), and sequence pairing represents complementarity predicted to influence binding efficacy. (D) Luciferase activity of MIB1 3′UTR wild-type reporter is reduced with miR-198 mimic while the mutant remains unchanged. Graph displaying a single representative experiment. The mean ± SEM and statistical significance are denoted (*P<0.05, ***P<0.001). MIB1, mindbomb E3 ubiquitin protein ligase 1.