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. 2019 Jul 16;42(3):1005–1016. doi: 10.3892/or.2019.7235

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Copyright: © Jou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — The RNA expression level and DNA CpG methylation level of WIF1 in 8 human urothelial cell lines. (A) The mRNA expression level of WIF1 was analyzed by RT-PCR assay. (B) The DNA methylation level of WIF1 was analyzed by BSP-MSP/USP assay. The MSP ratio (%)=MSP intensity/MSP intensity + USP intensity. Seven human bladder cancer cell lines (5637, J82, RT4, 1376, T24, TSGH8301, BFTC905) and one normal human urothelial cell line (SV-HUC1) were analyzed concurrently. (C) WIF1 mRNA expression after 5-aza-2′-deoxycytidine treatment for 3 days. WIF1, Wnt inhibitory factor 1; BSP, bisulfite-sequencing PCR; MSP, methylation-specific PCR; USP, unmethylation-specific PCR.