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. 2019 Jul 11;42(3):973–990. doi: 10.3892/or.2019.7230

Figure 8.

Figure 8.

CYP3A5 is a direct target gene of miR-543. (A) The screened target genes were initially validated in OSCC cell lines, and it was determined that the expression level of CYP3A5 had an inverse correlation with miR-543 in the OSCC cell lines. (B) The sequence of the miR-543 binding site within the 3′UTR of human CYP3A5. (C) CYP3A5 had a direct binding role with miR-543. Analysis of the relative luciferase activities of CYP3A5-WT and CYP3A5-MUT in 293T cells. The results revealed that overexpression of miR-543 significantly reduced the luciferase activity of WT CYP3A5 3′UTR, while the MUT CYP3A5 3′UTR had virtually no change. CYP3A5 (D) mRNA and (F) protein expression were decreased in SCC9, SCC25 and CAL27 cells following treatment with miR-543 mimic when compared with the mimic NC. CYP3A5 (E) mRNA and (G) protein expression were decreased in SCC9, SCC25 and CAL27 cells following treatment with the miR-543 inhibitor when compared with the inhibitor NC. *P<0.05, **P<0.01, ***P<0.001; ns, not significant; miR, microRNA; OSCC, oral squamous cell carcinoma; NC, negative control; CYP3A5, cytochrome P450 family 3 subfamily A member 5; UTR, untranslated region; MUT, mutant; WT, wild-type.