Table 3.

Process performance and product quality comparison of calcium phosphate precipitation process upon scale‐up from 1 mL to 1000 mL using Molecule 1 load i and from 1 mL to 2500 mL using Molecule 1 load ii calcium phosphate precipitation condition: 15 mM calcium chloride, 20 mM sodium phosphate

Load	Working Volume (mL)	Load Purity (% Mon, % agg)	Monomer Yield (%)	Product Purity (% Mon, % agg)
i	1	88.0, 12.0	89	96.0; 4.0
	1		89	96.5; 3.5
	1000		81	96.7; 3.3
	1000		85	96.6; 3.4
ii	1	91.4, 8.6	88	96.7; 3.3
	1		87	97.1; 2.9
	2500		86	96.6; 3.4
	2500		88	96.1; 3.9