Figure 5.

Pimozide treatment increases ROS generation in HCC cells. (A) Sk-Hep1 and Huh7 cells were treated with 10 µM pimozide, 2 mM NAC, or both for 48 h, and then stained with DCFH-DA. (A) Analysis of ROS production intensity among assayed cell populations. Representative images from one of three independent experiments are shown. (B) CCK-8 cell viability assay. Mean values from three independent experiments are shown with SDs, **P<0.01, compared to the control. ROS, reactive oxygen species; HCC, hepatocellular carcinoma; NAC, N-acetyl-l-cysteine.