Figure 3.

Enforced Notch1 expression rescues suppressed cell proliferation and invasion mediated by PrP silencing. A: Capan-1 cells were transfected with ICN1 (pEGFP-N1^IC), and immunoblotted to detect PrP and Notch1. B and C: The effect of enforced Notch1 expression in PrP-silenced cells (P-KD/N1-OE) on cell proliferation (B) and invasion (C) was compared with those in PrP-silenced cells (P-KD) and Ctrl cells. Data are expressed as means ± SD. n = 3 independent experiments (A); n = 5 biological replicates (B and C). t-test was performed (B and C). P values that are significantly different are shown as indicated. ^∗P < 0.05, ^∗∗P < 0.01. Ctrl, control; D1, 24 hours; D2, 48 hours; D3, 72 hours; ICN1, control plasmid or plasmid expressing activated Notch1; N1-OE, overexpression of Notch1; pEGFP, phosphorylated enhanced green fluorescent protein; P-KD, Prp knockdown; PrP, prion protein.