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. 2019 Jul 24;10:1752. doi: 10.3389/fimmu.2019.01752

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Copyright © 2019 Quinlan, Merino-Serrais, Di Grande, Dussmann, Prehn, Ní Chonghaile, Henshall and Jimenez-Mateos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CND protects neonates against the neuronal damage induced by hypoxia. P7 pups were exposed to normoxia or hypoxia and receive a single dose of vehicle (veh) or CND just after hypoxia. (A) Graph shows the number of silver-staining positive cells in the four experimental paradigms. Note: CND protects against hypoxia induced neuronal damage [n = 6–8, **p < 0.01, compared to control. F: 11.500, t: 5.141 (vs. control), t: 4.308 (vs. CND), t: 4.553 (vs. Hyp-CND)] (B) Representative high magnitude (40x) silver staining images from the hypoxia group. Scale bar: 10 μm. (C) Representative 10x silver staining images of the hilus 72 h post-treatment. Scale bar: 50 μm. (D) Representative 20x images of the hilus area of control, CND and/or hypoxia exposed mice. Scale bar: 20 μm.