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. 2019 Jul 24;10:938. doi: 10.3389/fphys.2019.00938

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Copyright © 2019 Gál, Dolenšek, Stožer, Pohorec, Ébert and Venglovecz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spatial displacement of ductal cells after stimulation with 1 mM CDCA. An intralobular duct is shown before (A) and during (B,C) the stimulation with chenodeoxycholic acid (CDCA). Constructs depicting early (B) and late (C) [Ca²⁺]_i response to CDCA display a background of cellular morphology in gray, and an overlay of calcium response in green (see Results section for detailed description). In some PDECs no spatial movement was detected (insets *), whereas in some cells noticeable spatial displacements were observed (insets ^†). Please note that in the PDECs that were displaced during CDCA response, change in [Ca²⁺]_i preceded the spatial displacement, therefore not interfering with the calcium response detection.