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. 2019 Jul 30;19:54. doi: 10.1186/s12896-019-0542-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Strategy for the construction of mtg and pro co-expression strains. This schematic map represents the constructed expression vectors for the pro and mtg, designated pGAP9/pro and pPICZα-rDNA/mtg, respectively. The his4 and rDNA non-coding sequences allow the vector to be inserted into the corresponding sites in the genome of strain GS115 through homologous recombination. The pPICZα-rDNA-mtg vector was constructed by introducing a non-coding rDNA (indicated as rDNAnc) sequence into the pPICZα vector. The expression of both pro and mtg were under the control of the GAP and AOX1 promoters, respectively