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. 2019 Jul 30;19:54. doi: 10.1186/s12896-019-0542-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Establishing balanced expression strains for pro-peptide and MTG. a The clones of GS115 (pro) were screened by Dot blot (anti-His-HRP).1–4 were four repeated of clones. b The protein (pro-peptide) of 20 μl culture supernatant was separated by Tricine-SDS-PAGE and stained with Coomassie blue under non-reducing conditions. c The co-expression of pro-peptide and MTG from screened colonies with various doses of Zeocin were experimented by Western blot under non-reducing conditions. d The enzyme activity of MTG from screened clones with various doses of Zeocin were experimented. Three individual clones were tested for each condition.***Represents a statistically significant difference of P < 0.001 compared with other two antibiotic dosage groups