Table 3.

PCR primers used in the present study

Primer name	Primer sequence (5′ → 3′)
P1	ACACTCGAGaaaagaGACTCCGACGACAGGGTCAC
P2	TATGCGGCCGCTCAATGGTGATGGTGATGATGCGGCCAGCCCTGC
P3	ACACTCGAGaaaagaGACAAT GGC GCG GGG GAAG
P4	TATGCGGCCGCTCAATGGTGATGGTGATGATGGGGAGCCCGGAACG
P5	TATGCGGCCGCCAGCTTTCTAGAACAAAAACTCATCTC
P6	CTTAAATATTAGGAAAAACGGTAACCTTATCTCACTTAATCTTCTGTACTCTG
P7	CAGAGTACAGAAGATTAAGAGAGATAGTTAGGTTACCGTTTTTCCTAATATTTAAG
P8	CGCGGATCCCTTCCACCAACAGTCAACCACCAGTC
P9	GGTATTAACGGTTTCGGACGTATTG
P10	GATGTTGACAGGGTCTCTCTCTTGG
P11	TGAAGAAAGAATTGGCTAACGG
P12	AGCTGGTCTGAAAGCATCTGG

Remarks: The underlined sites are those for the digestion of restriction enzymes XhoI. The wavy line sites are those for the digestion of restriction enzymes NotI. The double corrugated underlined sites are the ones for the digestion of restriction enzymes BamHI. 6xHis-tag label sequence is indicated by the dotted line. The Kex2-endopeptidase recognition site is marked with lowercase letters. The bold underlining represents the sequence of overlapping segments of the AOX1 terminator-rDNA fusion gene