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. 2019 Jul 31;39(31):6202–6215. doi: 10.1523/JNEUROSCI.2064-18.2019

Figure 5.

Figure 5.

Patch-clamp electrophysiology of ChR2-EYFP-positive and ChR2-EYFP-negative DRG neurons. A, Continuous exposure to 470 nm light for 1 s resulted in depolarization and light-evoked action potentials in ChR2-EYFP-positive DRG neurons (black) but not ChR2-EYFP-negative neurons (red). B, Exposure to light at 10 Hz drove phase-locked action potential firing in ChR2-EYFP-positive DRG neurons (black) but not ChR2-EYFP-negative controls (red). C, D, Success rate at 5 Hz and 10 Hz photostimulation of ChR2-EYFP-positive (n = 7) and ChR2-EYFP-negative (n = 4) neurons. E, Representative action potential waveforms from ChR2-EYFP-positive (top) and ChR2-EYFP-negative (bottom) DRG neurons. F, Width of the action potential waveform at half-maximum amplitude of ChR2-EYFP-positive and ChR2-EYFP-negative DRG neurons (Student's two-tailed t test, *p < 0.05). G, Action potential threshold of ChR2-EYFP-positive and ChR2-EYFP-negative DRG neurons (Student's two-tailed t test, **p < 0.005). H, Number of spikes under continuous photostimulation in ChR2-EYFP positive and ChR2-EYFP-negative neurons. C, D, and F–H are shown as means ± SEM.