Fig. 2.

The KLIpS could form a stable complex with HLA-A2 molecule. (A) 2 × 10⁵ TAP-deficiency T2 cells, incubated with either KLIpS (solid line), KLIS (dashed line), YML (light line, HLA-A2 binder) or VYV peptide (light-shaded, HLA-A11 binder) as indicated, were stained with PE-labeled anti-HLA-A2 Ab (BB7.2), and then monitored the MFI by flow cytometry. Cells without peptide incubation were as negative control (NC, dot line). Cells stained with isotype Ab are marked in dark Gray-fill. 1 × 10⁴ gating cells were analyzed. (B) The bar chart showed the relative MFI of peptide detected in T2 assays using the MFI of negative control as reference. Significant difference calculated with the Student’s t-test (two-tailed) and ANOVA is marked by asterisks. (^**, p < 0.01) The error bar represented the standard deviation (SD) of triplicate experiments.