Vaccination of KLIpS presented the anti-cancer effect to suppress the tumor growth. (A) AAD transgenic mice (n = 6) were inoculated with 2 × 105 TC1/AAD cancer cell for tumor development. Seven days after cancer cell injection, the tumor-bearing mice were immunized twice via footpad injection with either 50 μg KLIpS, KLIpS or PBS (control) formulated with PADRE in IFA on Day 7 and 14 indicated with arrow in figure. Tumor size was measured by palpation with calipers until the tumor volume exceeded 2000 mm3. (ANOVA test, KLIS vs KLIpS, **, p < 0.01). (B) Mice survival time was monitored and analyzed.