Supplementary Fig. S5.

Monitoring T cell induction in response to peptide immunization in transgenic mice by the ELISPOT assay. AAD transgenic mice (n=5) were immunized 50 μg peptide formulated with PADRE in IFA adjuvant. Seven days after final immunization, 5 x 105 lymph nodes were harvested and cocultured with peptide for 48 hrs in ELISPOT plate, and then inguinal lymph nodes were collected for detection of the IFN-γ-secreting cell population. Under antigen restimulation, adequate IFN-γ-secreting cells were detected in the lymph nodes of AAD transgenic mice that were immunized with KLIpS and YML (positive control), but they were rarely observed in mice immunized with KLIS and VYC (negative control). (Student t-test, KLIS vs KLIpS, **, p<0.01)