Figure 1. Ifit1 decreases MNV infection in RAW264.7 cells.

( A) Ifit1 knockout RAW264.7 cells were generated by CRISPR-Cas9 gene editing. Cells were stimulated with IFNβ for 12 hours then analysed by western blotting against Ifit1 and Ifit2/Ifit3. GAPDH was included as a loading control for each membrane. ( B, C) Infection of wild-type (WT) and Ifit1 knockout (KO) RAW264.7 cells at ( B) high or ( C) low multiplicity of infection (MOI) with murine norovirus (MNV-1). Viral titres were determined by 50% tissue culture infectious dose (TCID ₅₀) in BV2 cells and expressed as log ₁₀-transformed values. At late time points, indicated, severe cytopathic effect (cpe) was visible. Graphs show the mean and the standard error of three biological replicates. Titres were compared between WT and KO cells for each time point by two-tailed Student’s t-test. Asterisks indicate that a statistically significant difference (p < 0.05) was observed for both KO cell lines.