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. 2019 May 15;4:82. [Version 1] doi: 10.12688/wellcomeopenres.15223.1

Figure 2. Calicivirus translation is resistant to Ifit1 inhibition.

Figure 2.

( A) Schematic representations of in vitro transcribed cap0 genomic RNA or VPg-linked genomic and subgenomic RNAs, purified from infected cells, used for in vitro translation and toeprint assays. ( B) In vitro translation of cap0 or VPg-linked RNA from murine norovirus (MNV), porcine sapovirus (PSaV) and feline calicivirus (FCV). VP1, the dominant protein product produced from the VPg-linked subgenomic RNA, is indicated. ( C). Toeprint analysis of MNV, PSaV and FCV VPg-linked and cap0 RNA. Ifit1 binding is indicated by a cDNA product 6-7 nt shorter than the full-length signal (FL), indicated by black arrowheads. Red arrowheads indicate a 1-2 nt shorter full-length signal on VPg-linked RNAs.