Table I.
dpm/200 μL | mean ± SE | dpm/min/mg protein | % activity | |||
---|---|---|---|---|---|---|
control 0 min | 55 | 55 | 66 | 59 ± 4 | ||
mock (P-dol-) 10 min | 112 | 103 | 107 | 107 ± 3 | ||
mock RNAi 10 min | 1220 | 1326 | 1412 | 1319 ± 55 | 10,080 ± 420 | 100% |
algn-7 RNAi 10 min | 147 | 135 | 167 | 150 ± 9 | 801 ± 48 | 7.9% |
“control 0 min” indicates reaction mixture measured at time 0. “mock (P-dol-)” indicates reaction mixture without P-dolichol, and “mock RNAi” indicates RNAi with an empty vector.