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. 2019 Jul 24;10:931. doi: 10.3389/fphys.2019.00931

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Copyright © 2019 Kassa, Wood, Strader and Alayash.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of antisickling agent on the ODCs of AA intact cells. Suspensions of AA cell hematocrit (20%) were incubated in the presence of various concentrations of antisickling agent at 37°C for 15 min (TD-3) or 1 h (the other antisickling agents) prior to determination of the ODC using Hemox Analyzer. Oxygen dissociation curves (ODC) of AA cells with increasing molar ratio of TD-3, 5HMF, HU, and glutamine to Hb in heme are shown in (A), (B), (C), and (D), respectively.