Influenza HA-specific IgG concentrations after vaccination. The concentration of serum IgG specific to (a) the whole HA of H1N1pdm09 and (b) the stalk HA of H1N1pdm09 measured by ELISA. Four groups of healthcare workers (Group 1; G1, Group 2; G2, Group 3; G3 and Group 4; G4) were retrospectively selected according to their year of birth (G1; 1947–1952, G2; 1958–1960, G3; 1969–1972 and G4; 1978–1980). Serum samples were collected pre- and 21 days post vaccination. Each data point represents a single individual. The geometric mean titre (GMT) ± 95% confidence intervals are shown. *, p < 0.05; **, p < 0.01. # Indicates statistical significance (p < 0.05) from pre to post vaccination.