Figure 6.

In vivo replication kinetics. Shown are infectious viral titers in eyes (A), trigeminal ganglia (B), and brains (C) harvested from male and female mice infected with 17LpLz/VP16p² and the rescue of this mutant, 17LpLz/VP16p²-R. Three mice from each group were harvested at each time point indicated and infectious viral titers were quantified in tissue homogenates (see Section “Materials and Methods”). Brains were divided coronally into four pieces, labeled a–d from brainstem to front of brain as indicated in the diagram. * and ** indicate p = 0.01 < 0.05 and p = 0.001 < 0.01, respectively (unpaired, two-tailed t test).