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. Author manuscript; available in PMC: 2019 Jul 31.
Published in final edited form as: Bioorg Med Chem. 2017 Mar 23;25(12):2995–3005. doi: 10.1016/j.bmc.2017.03.048

Fig. 3.

Fig. 3.

Compounds up-regulate p21. (a) Detection of cyclin A2 and p21 levels over the course of 48 h as a result of treatment MDA-MB-468 cells. β-actin serves as loading control. (b) Quantification of data shown in (a). The data are presented as percentage changes (mean ± SD; n = 2). (c) Detection of cyclin A2 and p21 levels in MDA-MB-231 cells following treatment with 1 (KIN-1) and 6 (KIN-281) over 48 h. Actin is used as loading control. (d) Quantification of data in (c); the data are presented as percentage changes relative to control (mean ± SD; n = 2). (e) Detection of p53 phosphorylation and p53 levels following treatment of MDA-MB-468 cells over 48 h. Actin is used as loading control. (f) Effect of increasing concentration of 6 on MDMX levels detected by immunoblot analysis following treatment of MDA-MB-231.