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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Pain. 2019 Aug;160(8):1876–1882. doi: 10.1097/j.pain.0000000000001570

Figure 2. Vibration down-regulates expression of KV1.4 in DRG innervating the gastrocnemius muscle.

Figure 2.

A. Representative Western blots showing KV1.4 immunoreactivity in extracts from the ipsilateral L4-L5 DRGs of HAVS rats compared to control (naïve) rats; B. Quantitative analysis (ratio to reference protein) showed that, compared to controls, HAVS rats exhibited significantly decreased KV1.4 expression in DRG innervating the gastrocnemius muscle (unpaired t test, t = 1.859 df = 10, P = 0.046). β-actin was used as the housekeeping reference protein; C. KV1.4 knockdown in DRG by i.t. AS, but not SE, ODN treatment produced significant mechanical hyperalgesia in the gastrocnemius muscle. Two-way ANOVA showed significant effects for treatment (F1,10=192, P < 0.001), time (F4,40=123.1, P < 0.001) and interaction (F4,40=77.8, P < 0.001). Post hoc analysis of each time point revealed significant differences between AS and SE treated rats from days 1 to 7 after last ODN injection. *P < 0.05; ***P < 0.001.