Figure 2.

IFNs enhance TRIM14 transcription through ISRE. (A) pGL‐2020 or pGL‐121 was transfected into HeLa cells. After 32 h of transfection, the cells were treated with IFN‐α or IFN‐γ (10 ng·mL⁻¹) for 16 h. Luciferase activity was determined after 48 h transfection. (B, C) pGL‐2020 or pGL‐2020ISRE or pGL‐121 or pGL‐121mISRE was transfected into HeLa cells and HeLa cells were stimulated with IFN‐α or IFN‐γ (10 ng·mL⁻¹) 16 h before luciferase detection. Results are presented as mean ± SD, and data used for the analysis were from three independent experiments.