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. 2019 Jul 31;5:123. doi: 10.1038/s41420-019-0204-4

Fig. 4. LLC orthotopic tumor model on C57/BL mice with or without DKK2 knockdown.

Fig. 4

a, b C57/BL mice inoculated with LLC shControl and shDKK2 cells. Tumors were collected at day 19 and weighed (P < 0.05 for tumor growth at days 17 and 19, respectively; two-way ANOVA; two-tailed Student’s t test for tumor weight; n = 5). c Effects of DKK2 knockdown on tumor apoptosis by staining of the sections of cleaved caspase3. The sections were also counterstained with DAPI. Five independent sections per tumor and five tumors per group were examined (unpaired Student’s t test). Scale bars, 100 μm. Quantification of cleaved caspase3 staining was shown (right). Data are presented as mean ± s.e.m. d Granzyme B staining in tumor sections from shControl and shDKK2 groups. The sections were also counterstained with DAPI. Five independent sections per tumor and five tumors per group were examined (unpaired Student’s t test). Scale bars, 100 μm. Quantification of granzyme B staining was shown (right). Data are presented as mean ± s.e.m. (*p < 0.05; **p < 0.01)