Fig. 7. Anti-DKK2 antibody (5F8) suppresses tumor progress and activate immune effector cells in lungs of Kras^G12D; Apc^−/− mice.

a–c The mice (8 weeks old) were instilled intranasally with 65 μl of MEM containing adenovirus expressing Cre (2 × 10⁷ plaque-forming unites) and CaCl₂. After 6 weeks, the mice were treated weekly with IgG or 5F8 (10 mg/Kg). After another 6 weeks, some of the lungs (n = 4 IgG and n = 4 5F8) are sectioned, and five histological sections from a lung of a representative IgG or 5F8-treated lung are also shown in a. Scale bars, 100 μm. Quantification of total tumor number were shown in b, and comparison of tumor number in IgG or 5F8 groups after separating tumor as big tumors and small tumors based on tumor diameter (0.2 cm) (c). (Data are presented as means ± sem; two-sided Student’s t test). d–f The rest of the lungs (n = 4 IgG and n = 5 5F8) were analyzed by flow cytometry (e, g). d, f Flow cytometry analysis of tumor draining lymph nodes. MFI, mean fluorescence intensity. Data are presented as means ± sem (**p < 0.01 versus IgG; n = 4–5; two-sided Student’s t test)