Fig. 5.

Investigation of transcript identifiability. a, b Annotation status of junctions observed in each ONT and Illumina data set. A junction is considered observed if it is supported by at least 1 (a) or 5 (b) reads. For each observed junction, the distance to each annotated junction was defined as the absolute difference between their start positions plus the absolute difference between their end positions. This distance was used to find the closest annotated junction. c Distribution of the number of transcripts contained in the Salmon equivalence class that a read is assigned to, across all reads, for each ONT and Illumina data set. The center line represents the median; hinges represent first and third quartiles; whiskers the most extreme values within 1.5 interquartile range from the box. d As (c), but zoomed in to the range [0, 15]. The black diamond shape indicates the mean. Source data for panels a, b are provided as a Source Data file