Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 31;2:281. doi: 10.1038/s42003-019-0508-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — The lipid metabolic genes are frequently upregulated in ovarian cancer cells when cultured in OCM. a XTT cell proliferation assay demonstrates that treatment with OCM significantly increases cell growth in A2780cp, ES-2, SKOV3, and OVCA433 ovarian cancer cells. The relative cell viability was calculated by normalized to the mean value of day 1. b Transwell cell migration and c transwell cell invasion assays demonstrate that OCM treatment (12–24 h) promotes both cell migratory and invasive capacities in both OVCA433 and ES-2 cells. The stained cells were counted from four selected fields randomly. Representative images and quantitative results of cell migration and invasion were shown. Scale bar = 50 µm. d Ontology analysis on the altered genes detected by LC-MS/MS proteomic analysis indicates that most genes altered by OCM are associated with cellular and metabolic processes. The % of gene expression represents the % number of altered genes involved in each category of biological functions vs the total altered genes in ovarian cancer cells cultured in OCM as compared with the DMEM control. e A Venn diagram shows 31 out of 135 proteins related to metabolic processes are related to lipid metabolism. f Heatmap representation of the expression levels of the 31 proteins related to metabolism. g Ingenuity pathway analysis (IPA) depict interaction network of genes related to lipid metabolism in ovarian cancer cells. Results were presented as mean ± S.E.M. Data were analyzed by Student’s t-tests, and *p < 0.05 was considered as statistical significance