Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 31;2:281. doi: 10.1038/s42003-019-0508-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Ovarian cancer cells undergo lipogenesis in OCM. a The bar chart shows the Lipidomic analysis of the amount of intercellular unsaturated and saturated fatty acids in two ovarian cancer cells which were cultured in OCM from 0 to 24 h. The DMEM is used as a negative control. The Lipidomic analysis was performed by Metabo-Profile, Shanghai, China. b The percentage bar chart shows the changes of the major unsaturated fatty acid, Oleic acid, and two saturated fatty acids, Stearic acid and Palmitic acids in OVCA433 and ES-2 cells cultured in OCM from 0 to 24 h. c Western blot analysis compares the expression level of a key lipogenic enzyme, FASN, in two HOSEs and four ovarian cancer cell lines, SKOV3, OVCA433, Hey8, and ES-2. d Western blot analysis shows the reduction of FASN knockdown by lentiviral shRNAi approach in OVCA433 and ES-2. e Free fatty acid assay demonstrates the depletion of FASN leads to the reduction of long chain fatty acid (>8 carbon) in OVCA433 and ES-2 cells when cultured in DMEM or OCM. f Lipid droplet formation assay reveals the formation of lipid droplets in FASN knockdown OVCA433 and ES-2 cells as compared with their scrambled controls (SC). Scale bar = 50 µm. g XTT cell proliferation assay shows that 3 days of co-treatment with the above FASN inhibitors, orlistat (30 µM) and GSK2194069 (100 nM) (GSK), or AMPK activator, PF-06409577 (50 μM) (PF), significantly reduces the OCM-induced cell growth rate in SKOV3 and OVCA433 cells. h XTT cell proliferation assay reveals that the cell proliferation of OVCA433 with or without FASN knockdown co-cultured in OCM for 4 days. i Transwell cell migration assay shows the cell migratory rate of OVCA433 with or without FASN knockdown co-cultured in OCM for 12 h. The stained cells were counted from three selected fields randomly. Representative images and quantitative results of cell migration were shown. Scale bar = 50 µm. Results were presented as mean ± S.E.M. Data were analyzed by Student’s t-tests or one-way/two-way ANOVA with Tukey’s post hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)