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. 2019 Jul 31;9:11147. doi: 10.1038/s41598-019-47566-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miRNA knockout accelerates in vitro pancreatic acinar ductal metaplasia. Mouse pancreatic acinar cells were plated onto collagen and the transdifferentiation occurred over 4 days. Bright field images were captured at Day 1 (A–D) and Day 4 (E–H) of culture for the wild type (A,E), miR-217 (B,F), miR-216a (C,G) and miR-216b (D,H) KOs. (I) Number of ducts after 4 days of culture in the wild type and individual knockout mice. RNA was isolated from the cultures at days 1 to 4 of the transdifferentiation and the expression of J. cytokeratin 19, K. amylase 2A and L. carboxypeptidase A2 was determined by qRT-PCR. Data are presented relative to 18S rRNA (mean ± SEM) and were normalized to the day 0 controls. *P < 0.05 (Student’s t-test).