Figure 2.

ST inhibits the phosphorylation of AMPK and ACC and blocks H₂O₂-induced autophagy concurrent with the downregulation of AMPK activity. 786-O and ACHN (a,b) cells were treated with ST (0–8 μM/L) or H₂O₂ (0.1, 1 mM/L; 2 h) for up to 4 h, and the cells were lysed and subjected to immunoblotting (8–13% PAGE) with the indicated antibodies. tERK1/2 was used as a loading control. The results were similar among experiments repeated at least three times. ACHN (c,e) and 786-O (d,f) cells were treated with H₂O₂ (ACHN: 0.5 mM/L; 786-O: 0.1 mM/L) with or without ST (8 μM/L), or AICAR (0.5 mM/L) in the presence or absence of CQ (ACHN: 10 μM/L; 786-O: 20 μM/L) for 2 h. Cells were lysed and subjected to immunoblotting (8–13% PAGE) with the indicated antibodies. Actin was used as a loading control. The results were similar among experiments repeated at least twice. (Actin: A).