Figure 7.

p62 deprivation reduces AMPK activity, and MEK/ERK inhibition suppresses the ST-induced autophagic flux. (a,b) 786-O cells were transfected with the p62 siRNAs (sc-29679) for 48 h. The lysates were analyzed by immunoblotting (8–13% PAGE) following ST treatment (8 μM/L) for 4 h in the presence or absence of CQ (20 μM/L). (c–f) 786-O cells were treated with ST (4 μM/L) or H₂O₂ (0.1 mM/L, 2 h) with or without U0126 (10 μM/L) up to 4 h in the presence or absence of CQ (20 μM/L). Cells were lysed and subjected to immunoblotting (8–13% PAGE) with the indicated antibodies. The results were similar among experiments repeated twice. (Actin: A)