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. 2019 Jul 31;9:11087. doi: 10.1038/s41598-019-47597-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MEK/ERK could regulate the expression of p62. 786-O cells (a–c) and HeLa cells (d) were treated with ST (4 μM/L) with or without U0126 (10 μM/L) up to 4 h in the presence or absence of CQ (20 μM/L). Cells were lysed and subjected to immunoblotting (8–13% PAGE) and co-immunoprecipitated (8–13% PAGE) with the indicated antibodies. The results were similar among experiments repeated twice. (L-Exp: long exposure; HC: heavy chain; Actin: A)