Fig. 1.

Transport activity of reconstituted purified ASCT2_wt (a) and ASCT2_C467R (b). Curves show the counterflow of external radioactively-labelled and internal unlabelled l-glutamine (squares, n = 6 for ASCT2_wt, n = 3 for ASCT2_C467R); counterflow of l-glutamine in presence of 0.6 mM DL-TBOA (upward triangle, n = 4); counterflow of l-aspartate (circles, n = 3 for ASCT2_wt, n = 5 for ASCT2_C467R); and counterflow of l-aspartate in presence of 0.5 mM DL-TBOA (downward triangles, n = 3). Data points and error bars represent means and s.e.m. from n biologically independent experiments. Small schemes depict proteoliposomes with internal and external compound compositions used in respective experiments. Source data are provided as a Source Data file