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. 2019 Jul 31;10(8):574. doi: 10.1038/s41419-019-1776-x

Fig. 4. The key effector GLI1 of Integrin αvβ3/ERK1/2 pathway regulates the stem cell-like phenotype in MCAs of GC cells.

Fig. 4

a Western blottingting showing the most pronounced effect of GLI1 silencing in the shGLI1#1 group in BGC823MCAs and SGC7901MCAs. b Western blottingting showing evident overexpression of GLI1 in BGC823MCAs and SGC7901MCAs transduced with overGLI1 lentivirus. c Increased tumor spheres forming ability in the overGLI1 group and decreased formating ability in the shGLI1#1 group compared to the control group (WT) in SGC7901 MCAs and BGC823 MCAs. Scale bar = 10 μm. d Higher colony forming ability in the overGLI1 group and lower colony forming ability in the shGLI1#1 group compared to the control group in SGC7901 MCAs and BGC823 MCAs. e Western blottingting showing downregulated stemness-related genes in the shGLI1#1 group but upregulated genes CD44, ALDH1A1, Oct4, Bmi1 and Nanog in the overGLI1 group compared to the control group in SGC7901 MCAs and BGC823 MCAs. f, g Decreased volume and weight of xenograft tumors in the shGLI1#1 group and increased volume and weight in the overGLI1 group compared to the control group in SGC7901 MCAs and BGC823 MCAs. Each bar in the figure represents the mean ± SEM of triplicates. *p < 0.05; **p < 0.01