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. 2019 Jul 31;10:3436. doi: 10.1038/s41467-019-11373-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Elevated mineral accrual, carbonate deposition, and reduced amide I:II ratio in Dmp1Cre.Efnb2^f/f cortical bone. a Regions of periosteal bone used for synchrotron-based Fourier-transform infrared microspectroscopy (sFTIRM) analysis. Note the double calcein label on the newly mineralized periosteum; regions 1–3 (15 μm² in size) denote bone areas of increasing maturity with increasing distance from the periosteal edge. b Averaged sFTIRM spectra from regions 1, 2, and 3 of all 12-week old female Dmp1Cre (w/w) and Dmp1Cre.Efnb2^f/f (f/f) tibiae; grey boxes show approximate regions of the amide I, amide II, phosphate, and carbonate peaks (see Method for details). c–f sFTIRM-derived mineral:matrix (c), carbonate:mineral (d), amide I:II (e), and collagen crosslinking (f) ratios in regions 1–3 of w/w and f/f tibiae. Data are represented as mean ± SEM, with individual values, n = 13/group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. region 1 of same genotype (bone maturation effect), ⁺p < 0.05, ⁺⁺p < 0.01 vs. w/w in the same region (genotype effect) by two-way analysis of variance (ANOVA)