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. 2019 Jul 31;10:3430. doi: 10.1038/s41467-019-10882-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Rhizopine biosynthesis and signalling in M. truncatula and barley roots. a, b Gas chromatography-mass spectrometry (GC-MS) total ion chromatograms (TICs) of extracts prepared from M. truncatula transgenic roots (a) and transgenic T₀ barley seedlings (b) transformed with empty vector (control) or IdhA-MosB together. Highlighted peaks indicate scyllo-inosamine 1 (orange), scyllo-inosose 7 (green) and myo-inositol 3 (dark blue). All chromatograms are representative of experiments repeated at least three independent times. Source data for GC-MS chromatograms are provided as a Source Data file in Supplementary Materials. c, d NightOwl images showing bioluminescence of Rlv3841/pOPS0046 rhizopine lux biosensor on the surface of M. truncatula transgenic roots (c) and T₀ barley seedlings (d) transformed with empty vector (control) or IdhA-MosB together (engineered). Numbers in top right corners indicate number of plants tested that showed significant levels of bioluminescence (scale bar, 1 cm). Colours represent luminescence intensity from 10 or 20 counts per second (cool/purple) to 150 counts per second (warm/red). Source data underlying a, b are provided as a Source Data file