Fig. 5.

ATCOPIA preferentially targets environmentally responsive genes. a GO term analysis of genes with ATCOPIA93 or ATCOPIA78 insertions in the epiRILs or other experimental settings, or in nature. b Genome browser view of RNA-seq coverage of three genes hit by ATCOPIA93 in epiRIL394. c. Nectar secretion in flowers of epiRIL394 at F9 and F17. A nectar droplet is only observed at F17 (circle). d Structure of the FLC locus with the position of the ATCOPIA78 insertion in accession Ag-0 (top). Relative expression level of FLC at 10 and 60 days after germination (DAG) in plants containing (red) or lacking (blue) the ATCOPIA78 insertion and grown under control conditions (ctl.) or subjected to heat stress (HS) at the seedling stage (bottom). Data are mean ± s.d. (n > 9 independent samples, one biological experiment) and statistical significance for differences was obtained using the MWU test. e. Flowering time (mean ± s.d.; n > 9 independent samples, two independent experiments) for the same plants as in d. f Monthly mean temperature at collection sites for Ag-0 and 25 accessions sharing the same FLC haplotype but lacking the ATCOPIA78 insertion. Source data of Figure panels d and e are provided as a Source Data file