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. 2018 Jan 29;39(7):1266–1282. doi: 10.1177/0271678X18755628

Figure 3.

Figure 3.

2′MeO6MF increases tonic, but not phasic GABAAR currents in layer 2/3 pyramidal neurons after stroke. Recordings from layer 2/3 pyramidal neurons 3 h after stroke in the presence of 2′MeO6MF revealed a significant increase in tonic inhibitory currents in mice that received an initial 30 mg/kg i.p. dose of 2′MeO6MF (n = 11) 1 h after stroke, when compared to vehicle-treated controls (n = 10 (a)). Horizontal bars indicate the application of either 2′MeO6MF or vehicle, or the GABAAR antagonist SR95531. Cells were voltage clamped at + 10 mV. Data are presented as box plot (whiskers: minimum and maximum; lines: median) for mean ± SD (b). No change in phasic inhibitory currents was observed in mice treated with 30 mg/kg i.p. dose of 2′MeO6MF 1 h after stroke, when compared to vehicle-treated controls ((c) and (d)). Median IPSC amplitudes were not different (without 2′MeO6MF: 46.39 ± 19.9 pA; with 2′MeO6MF: 45.48 ± 21.79 pA; data collected from 2700 events: (c)). Cumulative probability curves reveal no difference in median inter-event intervals, i.e. no difference in frequency of IPSCs – without 2′MeO6MF (112 ms), with 2′MeO6MF (119.6 ms (d)). ***=P < 0.001, compared with stroke + vehicle-treated controls. IPSC: ; 2′MeO6MF: 2′-methoxy-6-methylflavone.