Figure 3.

Blood–brain barrier (BBB) disruption. (a) IgG fluorescence shows BBB leakage in corpus callosum (left worse than right) and left anterior and middle cerebral artery border zone in a representative SHRSP on high-salt diet (JPD). Scale bar = 2 mm. (b) Average volume of IgG leakage, expressed as % of each hemisphere shows BBB disruption starts after two weeks on JPD (n = 6), becomes severe at the onset of neurological signs (SxO, n = 11), and completely disappears within 7–10 days after switching to regular diet and starting antihypertensive treatment (Rev, n = 4). Right common carotid artery ligation (RCCAO, n = 9) was associated with significantly less ipsilateral BBB leakage. *p < 0.05 JPD SxO vs. all other groups; †p < 0.05 right (R) vs. left (L) RCCAO+JPD SxO; two-way repeated measures ANOVA followed by Sidak’s (R vs. L) and Holm–Sidak’s (among groups) multiple comparisons tests. Each data point represents a single animal. (c) Average volume of IgG leakage expressed as % of hemisphere in individual section levels from SHRSP on JPD at SxO, with or without BCCAO.