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. 2019 Jul 24;10:1684. doi: 10.3389/fmicb.2019.01684

Figure 4.

Figure 4

Characteristics of PVX-m460 hybrids in the infiltrated leaves of N. benthamiana. (A) Schematic diagram of the constructs based on pGR107. 35S represents CaMV 35S RNA promoter and NOS is the nopaline synthase terminator. The dashed line indicates the PVX-based viral expression vector. The open boxes represent the PVX-encoded proteins, including RNA-dependent RNA polymerase (RdRP), triple gene block movement proteins (25, 12, and 8 K), and coat protein (CP). Expression of m460, NES-m460 or NLS-m460 is driven by a duplicated CP subgenomic promoter, which is shown as a blue box. (B) Symptoms on leaves infiltrated with PVX, PVX-m460, PVX-NES-m460, and PVX-NLS-m460 at 10 dpi. (C) Northern blot analysis of total RNA from the infiltrated leaves at 10 dpi. Bands corresponding to the PVX viral RNAs are indicated, and methylene blue stain of 28S rRNA was used as a loading control. Numbers represent means with SD, indicating the relative abundance of the gRNA normalized to that of PVX. The experiments were repeated at least two times with similar results.