Table 1.
Enzyme activities of GalE enzymes.
| UDP-Glc | UDP-Gal | UDP-GlcNAc | UDP-GalNAc | Note | Ref. | |
|---|---|---|---|---|---|---|
| bGalE | — | 158 | 147 | — | Specific activity (U/mg) at 1 mM UDP-sugar | 15 |
| hGalE | — | 33.8 | — | 26 | Specific activity (U/mg) at 0.41 mM UDP-Gal or 0.66 mM UDP-GalNAc | 27 |
|
hGalE (C307Y) |
1.21 | 0.02 | Relative activity compared with the wild type enzyme at 2 mM UDP-Gal or 1.89 mM UDP-GalNAc | 43 | ||
| eGalE | — | 23.9 | — | 0.003 | Specific activity (U/mg) at 0.41 mM UDP-Gal or 0.66 mM UDP-GalNAc | 27 |
|
eGalE (Y299C) |
— | 5.1 | — | 0.69 | ||
| tGalE | — | 5.9 | — | — | Specific activity (U/mg) at 20 mM UDP-Gal | 54 |
| WbpP | 0.124 | 0.188 | 120 | 271 | kcat (min−1). Km values were similar for all substrates (197~251 uM) | 55 |
—, Not determined.