Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 18;4(1):e000307. doi: 10.1136/bmjophth-2019-000307

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Gating strategy in flow cytometry analysis. (A) Identification of singlets using FSC-A versus FSC-H. (B) Subtyping of singlets into monocytes and lymphocytes using FSC-A versus SSC-A. (C) Isolation of CD4⁺/CD8⁺ lymphocytes using fluorochrome-coupled specific antibodies. (D) Isolation of CD14⁺ monocytes using fluorochrome-coupled specific antibodies. (E) A general example of quantification of chemokine receptor (eg, CCR1) positive cells using a fluorescence histogram and a negative isotype control with a threshold of 1%. FSC-A, front scatter area; FSC-H, front scatter height; SSC-A, side scatter area.