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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Mucosal Immunol. 2019 May 15;12(4):958–968. doi: 10.1038/s41385-019-0172-2

FIG 2. NLRP3 is required for RV-triggered inflammasome.

FIG 2.

Wild type C57BL/6 (WT) or NLRP3−/−mice were inoculated with sham or RV for 24 hours. A. Lung mRNAs were measured by qPCR (N=6 from two different experiments, mean±SEM, *different from sham, p<0.05, unpaired t test). B. Lungs were stained for NLRP3 (green), F4/80 (red) and nuclei (DAPI, black; bar, 50 μm). Average number of F4/80+ NLRP3+ cells per airway (N=6 from one experiment, mean ±SEM, *different from sham, p<0.05, unpaired t test.) C, D. Whole lungs collected from C57BL/6 and NLRP3−/−mice were homogenized within lysis buffer and subjected to Western blot. Group mean expression levels relative to β-actin are also shown. (N=6 from three different experiments, mean±SEM, *different from sham, †different from wild type, p<0.05, two-way ANOVA.) E. Effect of NLRP3−/−on IL1b mRNA (N=7 from two different experiments, mean±SEM, *different from WT sham, †different from WT RV, p<0.05, two-way ANOVA with Tukey multiple comparisons test). For clarity, individual data points are not shown for panel D.