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. 2019 Apr 12;64(6):E200–E209.

Figure 5.

Figure 5

Promotion of the development of intestinal organoids by IEC-specific Tsc2 ablation. (A) Isolated crypts were cultured in the Matrigel with EGF for 3 days as described in Materials and Methods. Scale bar 100 μm. (B) Areas of intestinal organoids cultured as for (A) were determined. The number of buds per organoid was also determined. Data are means ± SE for a total of 90 organoids per group in three separate experiments. *, P < 0.05. (C) Intestinal organoids cultured without EGF were stimulated with (+) or without (−) Rapamycin for 4 days. Scale bar 100 μm. (D) Areas of intestinal organoids cultured as for (C) were determined. The number of buds per organoid was also determined. Data are means ± SE for a total of 90 organoids per group in three separate experiments. *, P < 0.05.